By Peter W. Kalivas, Shigenobu Toda, M. Scott Bowers, David A. Baker (auth.), John Q. Wang (eds.)
New molecular applied sciences have reworked our knowing of the neurological impression of gear of abuse and opened many new probabilities for learn. In medications of Abuse: Neurological studies and Protocols, best scientists from around the world evaluate those novel study components and supply a state of the art number of up-to-date and middle options for the neurological examine of substances of abuse. defined in step by step element, those with ease reproducible protocols hide a large choice of coherent tools for amassing details on quantitative adjustments in protein and mRNA at either tissue and mobile degrees. there are lots of equipment for detecting unmarried and a number of changes in unmarried and a number of gene expression, for reading the practical roles of genes and proteins, for learning the discharge kinetics of striatal dopamine, and for the quantitative size of such neurotransmitters as acetylcholine. extra equipment are supplied for basic neural phone tradition practise, for an immunohistochemical solution to research mobile proliferation and differentiation, and for a tradition strategy to develop plausible neural progenitors. All participants have significant event with the protocols lined, and every protocol has been completely attempted and effectively proven within the laboratory. valuable notes for every process talk about tricks-of-the-trade, pitfalls to prevent, and troubleshooting.
cutting-edge and hugely useful, medicinal drugs of Abuse: Neurological reports and Protocols deals insightful reports of key present issues and a center selection of with no trouble reproducible innovations crucial for any neurobiological laboratory accomplishing both simple analyses or looking novel examine approaches.
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Additional resources for Drugs of Abuse: Neurological Reviews and Protocols
Loftis, J. M. and Janowsky, A. (2000) Regulation of NMDA receptor subunits and nitric oxide synthase expression during cocaine withdrawal. J. Neurochem. 75, 2040–2050. 24. , Monteggia, L. , and Wolf, M. E. (1999) Withdrawal from repeated amphetamine administration reduces NMDAR1 expression in the substantia nigra, nucleus accumbens and medial prefrontal cortex. Eur. J. Neurosci. 11, 3167–3177. 25. Schenk, S. and Partridge, B. (1997) Effects of acute and repeated administration of N-methyl-D-aspartate (NMDA) into the ventral tegmental area: locomotor activating effects of NMDA and cocaine.
32, 359–363. 52. Mao, L. and Wang, J. Q. (2001) Differentially altered mGluR1 and mGluR5 mRNA expression in rat caudate nucleus and nucleus accumbens in the development and expression of behavioral sensitization to repeated amphetamine administration. Synapse 41, 230–240. 53. Swanson, C. , Baker, D. , Worley, P. , and Kalivas, P. W. (2001) Repeated cocaine administration attenuates group I metabotropic glutamate receptor-mediated glutamate release and behavioral activation: a potential role for Homer.
3. Summary: PFC and Other Cortical and Limbic Regions Glutamate receptor expression in the PFC undergoes complex changes after drug administration is discontinued that depend on the withdrawal time and probably differ between cocaine and amphetamine, at least for NR1. In general, some results suggest that AMPA receptor subunit expression changes at early withdrawal times whereas NMDA receptor subunit expression is altered after longer withdrawals. Because relatively few studies have assessed glutamate transmission in the PFC of sensitized rats using electrophysiological or neurochemical approaches, it is difficult to assess the functional significance of observed changes.
Drugs of Abuse: Neurological Reviews and Protocols by Peter W. Kalivas, Shigenobu Toda, M. Scott Bowers, David A. Baker (auth.), John Q. Wang (eds.)